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NaC20140103_DS_Lolina? Human AD-MSC stem-Prom? supplement kit 3,for adherent culture,Xeno Free-Lolina

NaC20140103_DS_Lolina? Human AD-MSC stem-Prom? supplement kit 3,for adherent culture,Xeno Free

    詳細(xì)說明

    Lolina A/S

    Address: Sindalsvej 30 8240 Risskov Danmark 

                        Email: Info@lolina.dk

     Website: https://lolina.dk


    Product Specification

     

    Product name

    Lolina? Human AD-MSC stem-Prom? supplement kit 3,for adherent culture, Xeno Free

    Cat.No.

    NaC20140103

    Storage and shipping

    NaC20140103-A,B,CStore at -20. Once added to medium, store at 4℃, do not refreeze after thawing. Dry ice transportation.

    NaC20140103-D: 2-8℃.

     

    Product Description

    Lolina? stem-Prom? supplement kit 3 is a sterile products which contains growth factors, hormones, or proteins. It mainly enhances the adhesion, proliferation and vitality of stem cells while maintaining their stemness. The kit is usually for adherent culture of AD-MSC in vitro.

    This kits has the following benefit:

    Enhance cell attachment and expansion ability: NaC20140103 can promote cell attachment and expansion on the culture matrix and enhance the interaction between cells and the matrix.

    Improve cell survival and proliferation rate: Adding NaC20140103 can significantly improve the survival and proliferation rate of MSCs, especially under low serum or serum-free culture conditions.

    Support cell differentiation potential: NaC20140103 can not only maintain the proliferation of MSCs, but also support their multidirectional differentiation potential.

    Provide an alternative to serum-free culture: NaC20140103, as a serum-free culture additive, can provide a safer and more controllable cell culture environment and reduce the dependence on FBS.

     

    Components

    Compound No.

    Compounds

    Size

    Format

    NaC20140103-A

    r-Human   serum albumi (HSA)

    62.5mg

    Lyophilized

    NaC20140103-B

    r-Huamn   bFGF protein

    2mg

    Lyophilized

    NaC20140103-C

    r-Human Fibronectin Protein

    2.5mg

    Lyophilized

    NaC20140103-D

    SafeGluta?

    2.17g

    Powder

     

    Instructions for Use

     

    1. Stock solution Preparation.

    One kit is for 50ml cell culture medium.

    The compounds are offered as powder in tubes. Please centrifuge before opening the cap to ensure the accuracy of the dosage.

    Please carry out dissolution and packaging operations on a clean bench.

    Spray the medium bottle and supplement tube with 70% ethanol and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior  threads  with  fingers. 

    Reconstitute NaC20140103-A, B, C in sterile 0.9% NaCl solution. Aliquot into appropriate volumes of storage solution. The recommend volume is 1 mL each. Aliquot into appropriate volumes of storage solution. When stored at -20℃, the stock solution is stable for 6 month. When stored at 4 ℃, the stock solution is stable for 1 week.

    Reconstitute NaC20140103-D in sterile 0.9% NaCl solution. The recommend volume is 5 mL. Aliquot into appropriate volumes of storage solution. When stored at 2-8 , -20℃, the stock solution is stable for 24 month.

     

    2. Reference Protocol

    Step 1: Culture medium Preparation

    a.  Thaw stock solutions of Lolina? Human AD-MSC stem-Prom? supplement kit 3.

    b.  Dilute the stock solutions into regular culture medium without L-Glutamine to get complete culture medium.  

    Step 2: Cell culture

    c.   Rapidly thaw frozen AD-MSC vials in a 37°C water bath.

    d.  Transfer cells to a sterile conical tube with pre-warmed medium.

    e.   Centrifuge at 300g for 5 minutes to pellet cells.

    f.   Resuspend the cell pellet in complete culture medium plate into culture dishes/flasks.

    g.   Change the medium every 2-3 days.

     

    Note

    Cell Density: Avoid over-confluence to maintain MSC multipotency.

    Medium Preparation: Always prepare fresh medium, even though SafeGluta? enhances stability.

    Contamination Control: Use aseptic techniques to prevent contamination.


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