詳細說明

Lolina A/S

Address: Sindalsvej 30 8240 Risskov Danmark 

                    Email: Info@lolina.dk

Product Specification

Product description

Universal Multiplex qPCR Master Mix (Probe Based) is a pre-solution for 2 × real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition. This product is a 2 × Mix pre-mixed reagent  that  enables  up  to  four fluorescent  quantitative  PCR  reactions  in  a  single reaction well. This product contains the genetically modified antibody method to hot- start Taq enzyme, greatly improving the amplification  sensitivity and specificity. At the same  time,  this  product  has  deeply  optimized  the  multi-reaction buffer, which  can improve  the   amplification   efficiency   of the  reaction   and  promote   the effective amplification of low-concentration templates. This product can be used for genotyping and multiplex quantitative analysis.

Instructions

1.Reaction S  stem

[Note]: Mix thoroughly before use to avoid excessive bubbles from vigorous shaking.

a)  Primer concentration: Primer Mix contains multiple pairs of primers, usually each primer at a final concentration of 0.2 μmol/L and can also be adjusted between 0. 1 and 0.5 μmol/L as appropriate.

b) Probe    concentration:    Probe    Mix     contains    multiple    probes    with     different fluorescence signals, and the concentration of each probe can be adjusted between  50 and 250nmol/L according to specific situation.

c)    Rox  dye  reference:  It  is  used  on  Real  Time  PCR  amplification  instrument   such   as  Applied Biosystems to correct the error of fluorescence signal generated between wells; this product does not contain Rox dye reference.

d) Template dilution: qPCR is highly  sensitive, and it is recommended to dilute the template for use. If the template is a cDNA stock solution, the template volume  should not exceed  1/10 of the total volume.

e)  Reaction system: 25 μL, 30 μL or 50 μL is recommended to ensure the effectiveness and repeatability of target gene amplification.

f)   System preparation: Please prepare in the super clean bench, and use the tips and reaction tubes without  nuclease  residue;   it  is  recommended  to  use  the  tips  with   filter  cartridges.  Avoid  cross contamination and aerosol contamination.

[Note]:

a)  Annealing/Extension:  The  temperature  and  time   can  be   appropriately adjusted according to the designed primer Tm value.

b)  Fluorescence  signal  acquisition:  The  fluorescence  signal  acquisition  time  required for   different qPCR  detection  instruments  is  different,  please  set  according  to  the minimum time limit. The time of several common instruments is set as follows:

20 sec: Applied Biosystems TT00, T900HT, T500 Fast

31 sec: Applied Biosystems 7300

32 sec: Applied Biosystems 7500

Notes

1.         Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety!

2.         This product is for scientific research purposes only.

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