詳細說明
Address: Sindalsvej 30 8240 Risskov Danmark
Email: Info@lolina.dk
Website: https://lolina.dk
Product Specification
Product name | Lolina? Mesenchymal Stem Cell Osteogenic Differentiation Supplement |
Cat.No. | NaC202201 |
Size | 5ml |
Storage and shipping | Store MODS at -20oC; once added to medium, store at 4oC and protect from light. Do not refreeze after thawing. Dry ice. |
Product Description
Lolina? Mesenchymal Stem Cell Osteogenic Differentiation Supplement (MODS) is a sterile, concentrated (100X) medium supplements designed for differentiation of primary mesenchymal stem cells (MSC) to an osteogenic lineage as determined by Alizarin Red staining in vitro. They are sterile, concentrated solutions that contain growth factors, hormones, and proteins necessary for MSC differentiation. The supplement is designed as additives for mesenchymal stem cell osteogenic differentiation medium (MODM) and should be used in conjunction with that medium.
Prepare for use
Thaw MODS at 37oC. Gently tilt the tubes several times during thawing to help the contents dissolve. Make sure the contents of the supplements are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add MODS and other components (FBS and P/S solution) into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of MODM are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
Note
MODS are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
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