詳細說明
Address: Sindalsvej 30 8240 Risskov Danmark
Email: Info@lolina.dk
Website: https://lolina.dk
Product Specification
Product name | Lolina? Osteoblast Mineralization Supplement |
Cat.No. | NaC201201 |
Size | 5ml |
Storage and shipping | Store ObMS at -20oC before addingto basal medium.Dry ice. |
Product Description
Lolina? Osteoblast Mineralization Supplement (ObMS) is a medium supplement designed for mineralization of primary osteoblasts as determined by Alizarin Red staining in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for osteoblast differentiation. The supplement is designed as an additive for Osteoblast Mineralization Medium (ObMM) and should be used in conjunction with that medium.
Prepare for use
Thaw ObMS at 37oC. Gently tilt the tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add ObMS and other components (FBS and P/S solution) into the basal medium in a sterile field. Mix well and the reconstituted medium is ready for use. Since several components of ObMM are light- labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
Note
ObMS is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
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