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NaM201004-2_2×Lolina Robust PCR Master Mix (With Dye)-Lolina

NaM201004-2_2×Lolina Robust PCR Master Mix (With Dye)

    詳細(xì)說明

    Lolina A/S

    Address: Sindalsvej 30 8240 Risskov Danmark 

                        Email: Info@lolina.dk

     Website: https://lolina.dk





    Product Specification

     

     

    Product name

    2×Lolina? Robust PCR Master Mix (With Dye)

    Cat.No.

    NaM201004-2

    Size

    1 mL/5×1 mL/100×1   mL

    Storage and shipping

    The product is shipped with dryice and can be stored at -20℃ for

    2 year.

     

    Product description

     

    2×Lolina?  Robust  PCR  Master  Mix  (With  Dye)  uses  modified  Taq  DNA  Polymerase,  adding powerful extension factors, amplification enhancement factors and an optimized buffer system. The amplification speed and amplification yield are a qualitative leap compared to ordinary PCR Mix. . The amplification  speed  can  reach   15  sec/kb,  which  is  suitable  for  fast  PCR  reactions.  The  ultimate amplification speed within 1 kb can reach 5 sec/kb, which greatly saves PCR reaction time. The premix contains dNTP and Mg2+ . When using it, you only need to add primers and templates for amplification, which greatly simplifies the experimental steps. The Mix contains electrophoresis indicator dye, which can be used for electrophoresis directly after the reaction is completed, making it easy to use. The protective agent added to the system allows the Master Mix to maintain stable activity after repeated freezing and thawing. The 3 ’ end of the PCR product contains A and can be easily cloned into a T vector.

    Instructions

     

    1. Recommended PCR Reaction System (50  μL)

     

    Components

    Volume μL

    2 ×Lolina? HotStart PCR Genotyping Master Mix (With Dye)

    25

    Template

    x

    Forward Primer   (10 μmol/L)

    2.5

    Reverse Primer   (10 μmol/L)

    2.5

    ddH20

    Up to 50

     

    2. Reaction program


    Cycle step

    Temp.

    Time

    Cycles

    Initial denaturation

    94 °C

    3-5 min

    1

    Denaturation

    94 °C

    10 sec

     

    30-35

    Annealing

    55 °C

    20 sec

    Extension

    72 °C

    15-30 sec/kb

    Final extension

    72 °C

    5 min

    1

    [Note]:  Please be sure to mix it thoroughly before use.

    1) Template usage amount: human genomic DNA: 30-100 ng; E. coli genomic DNA: 10-100 ng; λDNA: 0.5-5 ng; plasmid DNA: 0.1-10 ng.

    2) Mg2+ concentration: This product contains 3 mM MgCl2, which is suitable for most PCR reactions.

    3) Annealing temperature: Please refer to the theoretical Tm value of the primer. The annealing temperature can be set 1-2°C lower than the theoretical value of the primer.

    4) Extension speed: 5-15 sec/kb can be selected for genes within 1 kb in length.

     

    Notes

     

    1. This product is for research use only.

    2. Please operate with lab coats and disposable gloves for your safety.



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